Medium data

Search for medium no. = [1061].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1061 HALOBACTERIA MH4.2 MEDIUM

Sodium pyruvate	`1.0`	g
Trisodium citrate·2H₂O	`1.0`	g
Yeast extract (BD-Difco)	`2.0`	g
Tryptone (BD-Difco)	`2.0`	g
K₂HPO₄	`0.6`	g
NH₄Cl	`0.6`	g
NaCl	`200.0`	g
MgSO₄·7H₂O	`0.5`	g
K₂SO₄	`5.0`	g
Trace element solution (see Medium No. 167)	`1.0`	ml
FeCl₂·4H₂O	`36.0`	mg
MnCl₂·4H₂O	`0.36`	mg
0.5 M Tris-HCl solution (pH 8.0)	`100.0`	ml

Add components to distilled water and bring volume to 1.0 L. For preparation of solid medium, add 20.0 g agar.