Medium data

Search for medium no. = [1059].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1059 PYGV MARINE MEDIUM (B)

Bacto peptone (BD-Difco)	`0.25`	g
Yeast extract (BD-Difco)	`0.25`	g
Mineral salt solution (see Medium No. 304)	`20.0`	ml
2.5% Glucose solution	`10.0`	ml
Vitamin solution (see Medium No. 304)	`10.0`	ml
Artificial seawater (see Medium No. 304)	`250.0`	ml
Distilled water	`710.0`	ml

Mix components thoroughly except the glucose solution and Vitamin solution. Gently heat and bring to a boil. Autoclave and then cool to 45 - 50C. Aseptically add the filter-sterilized glucose solution and Vitamin solution. Adjust pH to 7.2 - 7.4 with sterile KOH, if necessary. For preparation of solid medium, add 15 g/L agar.