Medium data
Search for medium no. = [1048].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1048 METHANOGEN HIGH SALT MEDIUM
NaCl | 23.4 | g |
MgCl2·6H2O | 11.0 | g |
NaHCO3 | 3.8 | g |
NH4Cl | 1.0 | g |
KCl | 1.0 | g |
KH2PO4 | 0.68 | g |
CaCl2·2H2O | 0.3 | g |
Wolfe's mineral solution (see Medium No. 265) | 10.0 | ml |
Trace vitamins (see Medium No. 197) | 10.0 | ml |
Resazurin | 1.0 | mg |
Sodium acetate | 1.0 | g |
Yeast extract | 2.0 | g |
Distilled water | 1.0 | L |
Mix components, except NaHCO3, and adjust pH to 7.0. Bring to a boil for 5-10 sec, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. After autoclaving, stand the medium overnight. Prior to use, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
50% (v/v) Methanol solution | 10.0 | ml |
5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
5% Na2S·9H2O solution | 2.0 | ml |
Comment: For strain JCM 30226, supplement with 20 mM (final) tetramethylammonium as a substrate instead of methanol.
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