Medium data

Search for medium no. = [1048].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1048   METHANOGEN HIGH SALT MEDIUM

NaCl  23.4     g
MgCl2·6H2O  11.0     g
NaHCO3  3.8     g
NH4Cl  1.0     g
KCl  1.0     g
KH2PO4  0.68    g
CaCl2·2H2O  0.3     g
Wolfe's mineral solution (see Medium No. 265)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Resazurin  1.0     mg
Sodium acetate  1.0     g
Yeast extract  2.0     g
Distilled water  1.0     L
Mix components, except NaHCO3, and adjust pH to 7.0. Bring to a boil for 5-10 sec, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. After autoclaving, stand the medium overnight. Prior to use, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
50% (v/v) Methanol solution  10.0     ml
5% L-Cysteine·HCl·H2O solution  10.0     ml
5% Na2S·9H2O solution  2.0     ml
Comment: For strain JCM 30226, supplement with 20 mM (final) tetramethylammonium as a substrate instead of methanol.