Medium data

Search for medium no. = [1043].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NH4Cl  1.0     g
KH2PO4  0.3     g
K2HPO4  0.3     g
NaCl  25.0     g
KCl  0.1     g
CaCl2·2H2O  0.1     g
Na2SO4  4.0     g
Yeast extract (BD-Difco)  1.0     g
Trace element solution (see Medium No. 439)  1.0     ml
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Distilled water  920.0     ml
Mix ingredients and adjust pH to 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
1 M Sodium lactate  20.0     ml
15% MgCl2·6H2O solution  20.0     ml
5% NaHCO3 solution*  40.0     ml
Prior to use, reduce the medium by the addition of the following solution (autoclaved and stored under N2, per liter):
5% Na2S·9H2O solution  8.0     ml

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