Medium data
Search for medium no. = [1041].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1041 THIOBACILLUS PROSPERUS FO1 MEDIUM
| KCl | 0.33 | g |
| MgCl2·6H2O | 2.75 | g |
| NH4Cl | 1.25 | g |
| CaCl2·2H2O | 0.14 | g |
| KH2PO4 | 0.14 | g |
| K2HPO4 | 0.14 | g |
| NaCl | 20.0 | g |
| Distilled water | 940.0 | ml |
Adjust pH to 6.0 and autoclave. After cooling, aseptically add the following solutions (filter-sterilized):
| Trace mineral solution (see below) | 10.0 | ml |
| FeSO4·7H2O solution (see below) | 50.0 | ml |
Trace mineral solution:
| Trace minerals (see Medium No. 151) | 1.0 | L |
| NiCl2·6H2O | 0.225 | g |
| Na2SeO3·5H2O | 0.3 | g |
FeSO4·7H2O solution:
| FeSO4·7H2O | 2.0 | g |
| 0.1 N H2SO4 | 100.0 | ml |
Prepare just before use.
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