Medium data

Search for medium no. = [1039].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


KH2PO4  0.5     g
MgSO4·7H2O  0.4     g
NaCl  0.4     g
NH4Cl  0.4     g
CaCl2·2H2O  0.05    g
FeSO4·7H2O  2.0     mg
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Brain heart infusion (BD-Difco)  6.0     g
Proteose peptone (BD-Difco)  6.0     g
Yeast extract  2.0     g
Sodium acetate  1.0     g
Sodium formate  2.0     g
NaHCO3  4.0     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components, except NaHCO3 and vitamin solution, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels under a stream of H2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. Prior to inoculation, add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere).
Vitamin solution* (see below)  1.0     ml
5% Na2S·9H2O solution  10.0     ml
5% L-Cysteine·HCl·H2O solution  10.0     ml
After growth has started and the culture is becoming turbid, pressurize to 50-100 kPa H2-CO2 (80:20, v/v).
Vitamin solution:
Vitamin B12  100.0     mg
p-Aminobenzoic acid  80.0     mg
Biotin  20.0     mg
Nicotinic acid  200.0     mg
DL-Calcium pantothenate  100.0     mg
Pyridoxine·HCl  300.0     mg
Thiamine·HCl  200.0     mg
Distilled water  1.0     L

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