Medium data

Search for medium no. = [1037].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1037   MPYCY AGAR MEDIUM

Maltose monohydrate  0.5     g
Sodium pyruvate  0.4     g
Casamino acids (BD-Difco)  0.3     g
Yeast extract (BD-Difco)  0.3     g
Solution A (see Medium No. 1036)  5.0     ml
Solution B (see Medium No. 1036)  8.0     ml
Agar (if necessary)  12.0     g
Distilled water  1.0     L
Before autoclaving, adjust pH to 7.6 - 7.8 for agar plate and to 7.4 - 7.6 for liquid medium. Autoclave at 115C for 20 min. Separately sterilize Solution B by filtration and add to the medium aseptically.