Medium data

Search for medium no. = [1037].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1037 MPYCY AGAR MEDIUM

Maltose monohydrate	`0.5`	g
Sodium pyruvate	`0.4`	g
Casamino acids (BD-Difco)	`0.3`	g
Yeast extract (BD-Difco)	`0.3`	g
Solution A (see Medium No. 1036)	`5.0`	ml
Solution B (see Medium No. 1036)	`8.0`	ml
Agar (if necessary)	`12.0`	g
Distilled water	`1.0`	L

Before autoclaving, adjust pH to 7.6 - 7.8 for agar plate and to 7.4 - 7.6 for liquid medium. Autoclave at 115C for 20 min. Separately sterilize Solution B by filtration and add to the medium aseptically.