Medium data

Search for medium no. = [1036].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1036   EGGC LIQUID MEDIUM

Glucose  0.6     g
Sodium acetate  0.3     g
Casamino acids (BD-Difco)  0.3     g
CaCO3  0.02    g
Solution A (see below)  5.0     ml
Solution B (see below)  8.0     ml
Distilled water  1.0     L
Adjust pH to 7.0 - 7.2. Autoclave at 115C for 20 min. Separately sterilize Solution B by filtration and add to the medium aseptically.
Solution A:
(NH4)2SO4  20.0     g
MgSO4·7H2O  10.0     g
KCl  10.0     g
CaCl2·2H2O  4.0     g
Distilled water  1.0     L
Solution B:
0.5 M Na2HPO4-NaH2PO4 buffer (pH 7.3)  2.0     ml
Vitamins mix solution (see Medium No. 296)  5.0     ml
FeCl3 solution (see below)  1.0     ml
Mix and filter-sterilize just before addition to the medium.
FeCl3 solution:
FeCl3·6H2O  0.15    g
Distilled water  300.0     ml
Prepare just before use.