Medium data

Search for medium no. = [296].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

296   EGGC MEDIUM

Glucose  0.6     g
Sodium acetate  0.3     g
Casamino acids (BD-Difco)  0.3     g
(NH4)2SO4  0.1     g
MgSO4·7H2O  0.05    g
KCl  0.05    g
CaCl2·2H2O  0.02    g
CaCO3  0.02    g
Solution A (see below)  4.5     ml
Agar  12.0     g
Distilled water  1.0     L
Adjust pH to 7.2-7.4. Autoclave at 115C for 20 min. Separately sterilize Solution A by filtration, and add to the medium aseptically.
Solution A:
0.5 M Na2HPO4-NaH2PO4 buffer (pH 7.3)  2.0     ml
Vitamins mix solution (see below)  0.5     ml
FeCl3 solution (see below)  2.0     ml
Vitamins mix solution:
Riboflavin  20.0     mg
Thiamine·HCl  20.0     mg
Nicotinic acid  20.0     mg
Calcium pantothenate  20.0     mg
myo-Inositol  20.0     mg
p-Aminobenzoic acid  20.0     mg
Pyridoxine·HCl  20.0     mg
Folic acid  1.0     mg
Vitamin B12  1.0     mg
Biotin  1.0     mg
Distilled water  1.0     L
FeCl3 solution:
FeCl3·6H2O  0.15    g
Distilled water  300.0     ml
Prepare just before use.