Medium data
Search for medium no. = [1034].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1034 IRD SM40 MEDIUM
| Na2SO4 | 3.16 | g |
| NH4Cl | 1.0 | g |
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NaCl | 40.0 | g |
| KCl | 0.2 | g |
| CaCl2·2H2O | 0.1 | g |
| Sodium acetate | 0.5 | g |
| Sodium lactate | 6.0 | g |
| Yeast extract | 0.5 | g |
| Trace element solution (see Medium No. 439) | 1.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 955.0 | ml |
Mix components thoroughly and adjust pH to 7.5 with KOH. Bring to a boil, cool down under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N2 atmosphere or *filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture]:
| 15% MgSO4·7H2O solution | 20.0 | ml |
| 8% NaHCO3 solution* | 25.0 | ml |
| 5% Na2S·9H2O solution | 8.0 | ml |
Readjust pH to 7.5, if necessary.
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