Medium data
Search for medium no. = [1025].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1025 PYX MEDIUM
| Trypticase peptone | 5.0 | g |
| Bacto peptone (BD-Difco) | 5.0 | g |
| Yeast extract (BD-Difco) | 10.0 | g |
| Glucose | 2.0 | g |
| Resazurin | 1.0 | mg |
| Salt solution (see Medium No. 676) | 40.0 | ml |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Distilled water | 1.0 | L |
Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under a N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense into culture vessels under a N2 gas atmosphere and autoclave. After autoclaving, add glucose from a sterile, anoxic stock solution prepared under a N2 gas atmosphere.
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