Medium data

Search for medium no. = [1024].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1024   THERMUS 162 MEDIUM

Yeast extract (BD-Difco)  1.0     g
Tryptone (BD-Difco)  1.0     g
Nitrilotriacetic acid  0.1     g
CaSO4·2H2O  0.04    g
MgCl2·6H2O  0.2     g
0.01 M Iron(III) citrate solution  0.5     ml
Nitsch's trace elements (see Medium No. 273)  0.5     ml
Distilled water  900.0     ml
Adjust pH to 7.2 with NaOH. For preparation of solid medium, add 28.0 g/L agar. After autoclaving, add the following solution to the medium:
Phosphate solution (see below)  100.0     ml
Phosphate solution:
KH2PO4  5.44    g
Na2HPO4·12H2O  43.0     g
Distilled water  1.0     L
Adjust pH to 7.2.