Medium data
Search for medium no. = [273].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
273 TM BROTH MEDIUM
Polypepton (Nihon Pharm. Co.) | 4.0 | g |
Yeast extract (BD-Difco) | 2.0 | g |
NaCl | 1.0 | g |
Castenholz basal salt solution (see below) | 10.0 | ml |
Distilled water | 1.0 | L |
Adjust pH to 7.2.
Castenholz basal salt solution:
Nitrilotriacetic acid | 1.0 | g |
CaSO4·2H2O | 0.6 | g |
MgSO4·7H2O | 1.0 | g |
NaCl | 0.08 | g |
KNO3 | 1.03 | g |
NaNO3 | 6.89 | g |
Na2HPO4 | 1.11 | g |
FeCl3·6H2O solution (0.03%) | 10.0 | ml |
Nitsch's trace elements (see below) | 10.0 | ml |
Distilled water | 1.0 | L |
Adjust pH to 8.2.
Nitsch's trace elements:
H2SO4 | 0.5 | ml |
MnSO4·xH2O | 2.2 | g |
ZnSO4·7H2O | 0.5 | g |
H3BO3 | 0.5 | g |
CuSO4·5H2O | 0.016 | g |
Na2MoO4·2H2O | 0.025 | g |
CoCl2·6H2O | 0.046 | g |
Distilled water | 1.0 | L |
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