Medium data
Search for medium no. = [273].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
273 TM BROTH MEDIUM
| Polypepton (Nihon Pharm. Co.) | 4.0 | g |
| Yeast extract (BD-Difco) | 2.0 | g |
| NaCl | 1.0 | g |
| Castenholz basal salt solution (see below) | 10.0 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 7.2.
Castenholz basal salt solution:
| Nitrilotriacetic acid | 1.0 | g |
| CaSO4·2H2O | 0.6 | g |
| MgSO4·7H2O | 1.0 | g |
| NaCl | 0.08 | g |
| KNO3 | 1.03 | g |
| NaNO3 | 6.89 | g |
| Na2HPO4 | 1.11 | g |
| FeCl3·6H2O solution (0.03%) | 10.0 | ml |
| Nitsch's trace elements (see below) | 10.0 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 8.2.
Nitsch's trace elements:
| H2SO4 | 0.5 | ml |
| MnSO4·xH2O | 2.2 | g |
| ZnSO4·7H2O | 0.5 | g |
| H3BO3 | 0.5 | g |
| CuSO4·5H2O | 0.016 | g |
| Na2MoO4·2H2O | 0.025 | g |
| CoCl2·6H2O | 0.046 | g |
| Distilled water | 1.0 | L |
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