Medium data

Search for medium no. = [273].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

273   TM BROTH MEDIUM

Polypepton (Nihon Pharm. Co.)  4.0     g
Yeast extract (BD-Difco)  2.0     g
NaCl  1.0     g
Castenholz basal salt solution (see below)  10.0     ml
Distilled water  1.0     L
Adjust pH to 7.2.
Castenholz basal salt solution:
Nitrilotriacetic acid  1.0     g
CaSO4·2H2O  0.6     g
MgSO4·7H2O  1.0     g
NaCl  0.08    g
KNO3  1.03    g
NaNO3  6.89    g
Na2HPO4  1.11    g
FeCl3·6H2O solution (0.03%)  10.0     ml
Nitsch's trace elements (see below)  10.0     ml
Distilled water  1.0     L
Adjust pH to 8.2.
Nitsch's trace elements:
H2SO4  0.5     ml
MnSO4·xH2O  2.2     g
ZnSO4·7H2O  0.5     g
H3BO3  0.5     g
CuSO4·5H2O  0.016   g
Na2MoO4·2H2O  0.025   g
CoCl2·6H2O  0.046   g
Distilled water  1.0     L