1023 PYVL MEDIUM
| Salt solution I (see Medium No. 133) | 75.0 | ml |
| Salt solution II (see Medium No. 133) | 75.0 | ml |
| Trypticase peptone (BD-BBL) | 10.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| L-Lysine | 5.0 | g |
| Vitamin mixture (see below) | 1.0 | ml |
| 0.1% (w/v) resazurin-Na | 1.0 | ml |
| 8% (w/v) Na2CO3 | 2.5 | ml |
| L-Cysteine·HCl·H2O | 0.3 | g |
| Distilled water | 850.0 | ml |
| Biotin | 1.0 | mg |
| Vitamin B12 | 1.0 | mg |
| p-Aminobenzoic acid | 3.0 | mg |
| Folic acid | 5.0 | mg |
| Thiamine·HCl | 5.0 | mg |
| Riboflavin | 5.0 | mg |
| Pyridoxamine·2HCl | 15.0 | mg |
| Distilled water | 100.0 | ml |