Medium data

Search for medium no. = [1019].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1019 MMJHS-2 MEDIUM

Modified MJ synthetic seawater B (see Medium No. 892)	`1.0`	L
NaNO₃	`1.0`	g
Na₂S₂O₃·5H₂O	`1.0`	g
NaHCO₃	`1.0`	g
Sulfur (powder)	`10.0`	g

Mix components, except sulfur and NaHCO₃, and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize 8% (w/v) NaHCO₃ solution and add to the medium. Aseptically distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of the medium with 0.2 g sulfur in 100 ml serum bottle) and seal with butyl rubber stoppers. Replace gas phase with a H₂-CO₂ (4:1, v/v) gas mixture and check medium pH to be 6.0. Pressurize the inoculated bottles to 200 kPa H₂-CO₂ (4:1, v/v).