Medium data
Search for medium no. = [371].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
371 THERMOVIBRIO AMMONIFICANS MEDIUM
| Synthetic seawater (2 x, see Medium No. 244) | 250.0 | ml |
| NaCl | 6.15 | g |
| KH2PO4 | 0.5 | g |
| CaCl2·2H2O | 0.375 | g |
| (NH4)2SO4 | 10.0 | mg |
| KI | 0.025 | mg |
| Na2WO4·2H2O | 0.1 | mg |
| NiCl2·6H2O | 2.0 | mg |
| KNO3 | 1.0 | g |
| MES | 5.0 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 750.0 | ml |
Mix ingredients, except KNO3, MES and Na2S·9H2O, bring to boil, and cool under a H2-CO2 (4:1, v/v) gas mixture. Slowly add 5% Na2S·9H2O solution to the medium, wait for the medium to become colorless, then adjust pH to 5.5. Distribute medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers, and autoclave. Separately autoclave 10% KNO3 and 20% MES solutions (pH 5.5) under a N2 atmosphere. Prior to inoculation, add the sterile KNO3 and MES solutions. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v) gas mixture.
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