Medium data

Search for grmd=[151].

151 PYROCOCCUS MEDIUM

Salt base solution (see below)	`1.0`	L
Trace minerals (see below)	`10.0`	ml
Citric acid	`5.0`	mg
Yeast extract (Difco)	`1.0`	g
Bacto peptone (Difco)	`5.0`	g
Sulfur (powder)	`30.0`	g
Na₂S·9H₂O	`0.5`	g
Resazurin	`1.0`	mg

Mix ingredients, except sulfur and Na₂S·9H₂O, and adjust pH to 6.5 with H₂SO₄. Sterilize by filtration under a N₂ atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na₂S·9H₂O as a 5% solution, and autoclave under a N₂ atmosphere. Aseptically and anaerobically add sterile sulfur to the medium. Prior to inoculation, add the sterile Na₂S·9H₂O solution, and readjust pH to 6.5, if necessary.
Salt base solution:

NaCl	`13.85`	g
MgSO₄·7H₂O	`3.5`	g
MgCl₂·6H₂O	`2.75`	g
KCl	`0.325`	g
NaBr	`0.05`	g
H₃BO₃	`15.0`	mg
SrCl₂·6H₂O	`7.5`	mg
KI	`0.05`	mg
CaCl₂	`0.75`	g
KH₂PO₄	`0.5`	g
(NH₄)₂Ni(SO₄)₂·6H₂O	`2.0`	mg
Distilled water	`1.0`	L

Trace minerals:

Nitrilotriacetic acid	`1.5`	g
MgSO₄·7H₂O	`3.0`	g
MnSO₄·xH₂O	`0.5`	g
NaCl	`1.0`	g
FeSO₄·7H₂O	`0.1`	g
CoSO₄·7H₂0	`0.1`	g
CaCl₂·2H₂O	`0.1`	g
ZnSO₄·7H₂O	`0.1`	g
CuSO₄·5H₂O	`0.01`	g
AlK(SO₄)₂	`0.01`	g
H₃BO₃	`0.01`	g
Na₂MoO₄·2H₂O	`0.01`	g
Distilled water	`1.0`	L

Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.