Medium data

Search for medium no. = [206].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

206

Salt base solution (see below)	`1.0`	L
Trace minerals (see Medium No. 151)	`10.0`	ml
Citric acid	`5.0`	mg
Tryptone (BD-Difco)	`6.0`	g
Sulfur (powder)	`6.0`	g
Na₂S·9H₂O	`0.5`	g
Resazurin	`1.0`	mg

Mix ingredients, except sulfur and Na₂S·9H₂O, and adjust pH to 6.5 with H₂SO₄. Sterilize by filtration while gassing with H₂-CO₂ (80:20) gas mixture. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na₂S·9H₂O as a 5% solution, and autoclave under a N₂ atmosphere. Aseptically and anaerobically add sterile sulfur to the medium under a H₂-CO₂ (80:20) atmosphere. Prior to inoculation, add the sterile Na₂S·9H₂O solution. Pressurize inoculated bottles to 100 kPa H₂-CO₂ (80:20).
Salt base solution:
Use Salt base solution of Medium No. 151 with 17.0 g/L (final) NaCl and 2.5 mg/L (final) KI.