Medium data
Search for medium no. = [1209].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1209 HALANAEROBIUM HYDROGENIFORMANS MEDIUM
Solution A:
| NaCl | 70.0 | g |
| Na2CO3 | 40.0 | g |
| Distilled water | 500.0 | ml |
Solution B:
| K2HPO4 | 6.3 | g |
| Basal salts solution (see below) | 10.0 | ml |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Distilled water | 380.0 | ml |
Autoclave solutions A and B, separately, under a N2 gas atmosphere. After cooling, combine both solutions and add the following solution (autoclaved and stored under N2):
| 10% Yeast extract solution | 10.0 | ml |
| 25% (w/v) Glycerin solution | 100.0 | ml |
Aseptically and anaerobically, distribute the medium into culture vessels (e.g., 10 ml in Balch type tubes) under a N2 gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solutions (autoclaved and stored under N2):
| 5% L-Cysteine·HCl·H2O solution | 12.0 | ml |
| 5% Na2S·9H2O solution | 15.0 | ml |
Basal medium salts solution:
| NH4NO3 | 50.0 | mg |
| MgCl2·6H2O | 8.5 | mg |
| SiO2 | 7.5 | mg |
| MnSO4·xH2O | 4.5 | mg |
| CaCl2·2H2O | 4.2 | mg |
| Methylene blue | 4.0 | mg |
| FeSO4·7H2O | 1.8 | mg |
| Distilled water | 1.0 | L |
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