Medium data

Search for medium no. = [1166].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1166 DESULFOVIBRIO DECHLORACETIVORANS MEDIUM

KH₂PO₄	`0.25`	g
K₂HPO₄	`0.25`	g
NH₄Cl	`0.5`	g
NaCl	`10.0`	g
Na₂SO₄	`2.8`	g
MgCl₂·6H₂O	`0.5`	g
MgSO₄·7H₂O	`0.2`	g
CaCl₂·2H₂O	`0.005`	g
Trace metal solution SL12 (see Medium No. 576)	`1.0`	ml
Sodium lactate	`1.12`	g
Selenite-tungstate solution (see Medium No. 431)	`0.4`	ml
Resazurin	`1.0`	mg
Distilled water	`1.0`	L

Mix components thoroughly, distribute the medium into culture vessels under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`30.0`	ml
Vitamin solution* (see Medium No. 556)	`1.0`	ml
5% Na₂S·9H₂O solution	`4.0`	ml

Adjust pH to 7.1 - 7.2, if necessary.