556 METHYLOSOMA DIFFICILE MEDIUM
NaCl | 0.1 | g |
MgCl2·6H2O | 0.04 | g |
KCl | 0.05 | g |
CaCl2·2H2O | 0.015 | g |
Na2SO4 | 0.016 | g |
Distilled water | 993.0 | ml |
2 M HEPES buffer (pH 7.0) | 5.0 | ml |
1 M Phosphate buffer (pH 7.2, see below) | 0.15 | ml |
0.09 M KNO3 solution | 0.544 | ml |
FeCl2 solution (see Medium No. 187) | 1.0 | ml |
Trace element solution (see Medium No. 187) | 1.0 | ml |
Vitamin solution (filter-sterilized, see below) | 1.0 | ml |
Vitamin B12 | 50.0 | mg |
p-Aminobenzoic acid | 50.0 | mg |
Biotin | 10.0 | mg |
Nicotinic acid | 100.0 | mg |
DL-Calcium pantothenate | 25.0 | mg |
Pyridoxine·HCl | 250.0 | mg |
Thiamine·HCl | 50.0 | mg |
Distilled water | 1.0 | L |