Medium data
Search for medium name = [%MRS%].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1 MRS MEDIUM
| Casein peptone, tryptic digest | 10.0 | g |
| Beef extract (BD-Difco) | 10.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| Glucose | 20.0 | g |
| Tween 80 | 1.0 | g |
| K2HPO4 | 2.0 | g |
| Sodium acetate | 5.0 | g |
| Diammonium citrate | 2.0 | g |
| MgSO4·7H2O | 0.2 | g |
| MnSO4·xH2O | 50.0 | mg |
| Distilled water | 1.0 | L |
Adjust pH to 6.0-6.5.
111 MRS MEDIUM (pH 5.5)
| MRS medium (see Medium No. 1) | 1.0 | L |
Adjust pH to 5.5.
257 MRS MEDIUM WITH 10% NaCl
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| NaCl | 100.0 | g |
| Bacto agar (BD-Difco) | 15.0 | g |
| Distilled water | 1.0 | L |
291 MODIFIED MRS MEDIUM
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Distilled water | 1.0 | L |
Adjust pH to 6.5.
293 MRS MALTOSE MEDIUM
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| Maltose | 10.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 6.5.
491 1/2 MRS + 1/2 BHI AGAR
| Lactobacilli MRS broth (BD-Difco) | 27.5 | g |
| Brain heart infusion broth (BD-Difco) | 18.5 | g |
| Agar | 15.0 | g |
| Distilled water | 1.0 | L |
509 MRS MEDIUM WITH 5% NaCl
| Lactobacillus MRS broth (BD-Difco) | 55.0 | g |
| NaCl | 50.0 | g |
| Bacto agar (BD-Difco) | 15.0 | g |
| Distilled water | 1.0 | L |
596 MRS MEDIUM WITH 2.5% NaCl
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| NaCl | 25.0 | g |
| Bacto agar (BD-Difco) | 15.0 | g |
| Distilled water | 1.0 | L |
730 RYE-BRAN MRS
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| Rye-bran extract (see below) | 900.0 | ml |
| Distilled water | 100.0 | ml |
Rye-bran extract:
| Rye-bran | 40.0 | g |
| Malted wheat meal | 2.0 | g |
| Trypsin (Sigma-Aldrich) | 0.8 | g |
| Distilled water | 1.0 | L |
Mix components and incubate at 50C for 24 hr. Clarify the resulting turbid liquid by centrifugation (500 x g, 15 min) followed by paper-filtration.
853 MRS FRUCTOSE MEDIUM
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| Fructose | 10.0 | g |
| Distilled water | 1.0 | L |
925 MRS MEDIUM WITH SOYBEAN PEPTONE
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| Phytone peptone (BD-BBL) | 5.0 | g |
| Bacto agar (BD-Difco) | 15.0 | g |
| Distilled water | 1.0 | L |
931 MRS MEDIUM WITH 2.5% NaCl (pH 9.0)
| Lactobacilli MRS broth (BD-Difco) | 55.0 | g |
| NaCl | 25.0 | g |
| Bacto agar (BD-Difco) | 15.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 9.0.
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