Medium data

Search for medium no. = [996].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

996 CAR BACILLUS MEDIUM

Use the Vero E6 cell culture supernatant fluid (see below). Incubate the microorganism in the supernatant fluid in a polystyrene culture flask (75 cm² flask, canted neck, vent cap, coated with Ultra-Low Attachment Surface, Corning: Cat. No. 3814) at 37C under 5% CO₂.
Supernatant fluid:
Incubate Vero E6 cells (ATCC CRL-1586) in Iscove's Modified Dulbecco's Medium (IMDM, GlutaMAX^TM) (Life Technologies: Cat. No. 31980030) supplemented with 10% fetal bovine serum (FBS; inactivated at 56C for 30 min) at 37C under 5% CO₂. After cultivation, centrifuge the culture medium. Use the supernatant fluid for cultivation of the microorganism as described above.