Medium data

Search for medium no. = [995].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

995 M30

Solution 1:

Modified Hutner's basal salts (see Medium No. 900)	`20.0`	ml
Aged seawater	`700.0`	ml
0.1 M Tris-HCl buffer (pH 7.5)	`50.0`	ml
Distilled water	`260.0`	ml

For solid medium, add 5.0 g/L gelrite (Gellan Gum).
Solution 2:

N-Acetyl-D-glucosamine (Sigma)	`2.0`	g
Na₂HPO₄·2H₂O	`0.01`	g
Ampicillin (50 mg/ml)	`4.0`	ml
Vitamin solution No. 6 (see below)	`10.0`	ml
Distilled water	`40.0`	ml

Add 0.2 ml filter-sterilized Solution 2 to Solution 1.
Vitamin solution No. 6:

Filter-sterilize.