Medium data

Search for medium no. = [995].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

995   M30

Solution 1:
Modified Hutner's basal salts (see Medium No. 900)  20.0     ml
Aged seawater  700.0     ml
0.1 M Tris-HCl buffer (pH 7.5)  50.0     ml
Distilled water  260.0     ml
For solid medium, add 5.0 g/L gelrite (Gellan Gum).
Solution 2:
N-Acetyl-D-glucosamine (Sigma)  2.0     g
Na2HPO4·2H2O  0.01    g
Ampicillin (50 mg/ml)  4.0     ml
Vitamin solution No. 6 (see below)  10.0     ml
Distilled water  40.0     ml
Add 0.2 ml filter-sterilized Solution 2 to Solution 1.
Vitamin solution No. 6:
Biotin  4.0     mg
Pyridoxine·HCl  20.0     mg
Thiamine·HCl·2H2O  10.0     mg
Calcium pantothenate  10.0     mg
p-Aminobenzoic acid  10.0     mg
Folic acid  4.0     mg
Riboflavin  10.0     mg
Nicotinamide  10.0     mg
Vitamin B12  0.2     mg
Distilled water  1.0     L
Filter-sterilize.