Medium data

Search for medium no. = [985].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

985 METHANOMETHYLOVORANS UPONENSIS MEDIUM

NaCl	`1.0`	g
KCl	`0.5`	g
MgCl₂·6H₂O	`0.4`	g
CaCl₂·2H₂O	`0.1`	g
KH₂PO₄	`0.2`	g
NH₄Cl	`0.5`	g
Resazurin	`1.0`	mg
Distilled water	`960.0`	ml

Mix components thoroughly and autoclave under a N₂-CO₂ (80:20, v/v) gas atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):

Trace vitamins* (see Medium No. 197)	`10.0`	ml
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
3% Trimethylamine·HCl solution*	`10.0`	ml
8% NaHCO₃ solution*	`20.0`	ml

Aseptically and anaerobically distribute the medium into culture vessels under a N₂-CO₂ (80:20, v/v) gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N₂ atmosphere):

5% Na₂S·9H₂O solution 8.0 ml