Medium data

Search for medium no. = [985].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

985   METHANOMETHYLOVORANS UPONENSIS MEDIUM

NaCl  1.0     g
KCl  0.5     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.1     g
KH2PO4  0.2     g
NH4Cl  0.5     g
Resazurin  1.0     mg
Distilled water  960.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
Trace vitamins* (see Medium No. 197)  10.0     ml
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
30% Trimethylamine·HCl solution*  10.0     ml
8% NaHCO3 solution*  20.0     ml
Aseptically and anaerobically distribute the medium into culture vessels under a N2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution  8.0     ml