Medium data
Search for medium no. = [975].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
975 SALINIVIBRIO SHARMENSIS MEDIUM
| NaCl | 100.0 | g |
| Trisodium citrate | 3.0 | g |
| MgSO4·7H2O | 1.0 | g |
| KCl | 2.0 | g |
| MnCl2·4H2O | 0.36 | mg |
| FeSO4·7H2O | 0.05 | g |
| Yeast extract (BD-Difco) | 10.0 | g |
Add components to distilled water and bring volume to 970 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add the following solution (separately autoclaved):
| 10% (w/v) Na2CO3 solution | 30.0 | ml |
Check the final pH to be around 9.0.
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