Medium data

Search for medium no. = [965].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

965   G-S MEDIUM

Yeast extract (BD-Difco)  4.5     g
KH2PO4  1.5     g
K2HPO4  2.9     g
Urea  2.9     g
L-Cysteine·HCl·H2O  3.0     g
MOPS  20.9     g
Cellobiose  10.0     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly and adjust pH to 7.4. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter 1 ml following sterile solution from anaerobic stock.
Mineral solution:
MgCl2·6H2O  2.5     g
CaCl2·2H2O  0.375   g
FeSO4·7H2O  0.031   g
ZnCl2  10.0     mg
L-Cysteine·HCl·H2O  0.03    g
Distilled water  10.0     ml

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