Medium data
Search for medium no. = [965].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
965 G-S MEDIUM
| Yeast extract (BD-Difco) | 4.5 | g |
| KH2PO4 | 1.5 | g |
| K2HPO4 | 2.9 | g |
| Urea | 2.9 | g |
| L-Cysteine·HCl·H2O | 3.0 | g |
| MOPS | 20.9 | g |
| Cellobiose | 10.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 7.4. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter 1 ml following sterile solution from anaerobic stock.
Mineral solution:
| MgCl2·6H2O | 2.5 | g |
| CaCl2·2H2O | 0.375 | g |
| FeSO4·7H2O | 0.031 | g |
| ZnCl2 | 10.0 | mg |
| L-Cysteine·HCl·H2O | 0.03 | g |
| Distilled water | 10.0 | ml |
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