Medium data
Search for medium no. = [965].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
965 G-S MEDIUM
Yeast extract (BD-Difco) | 4.5 | g |
KH2PO4 | 1.5 | g |
K2HPO4 | 2.9 | g |
Urea | 2.9 | g |
L-Cysteine·HCl·H2O | 3.0 | g |
MOPS | 20.9 | g |
Cellobiose | 10.0 | g |
Resazurin | 1.0 | mg |
Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 7.4. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter 1 ml following sterile solution from anaerobic stock.
Mineral solution:
MgCl2·6H2O | 2.5 | g |
CaCl2·2H2O | 0.375 | g |
FeSO4·7H2O | 0.031 | g |
ZnCl2 | 10.0 | mg |
L-Cysteine·HCl·H2O | 0.03 | g |
Distilled water | 10.0 | ml |
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