Medium data
Search for medium no. = [964].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
964 480G MEDIUM
| NaCl | 0.5 | g |
| NH4Cl | 0.33 | g |
| CaCl2·2H2O | 0.15 | g |
| MgCl2·6H2O | 0.35 | g |
| KCl | 0.3 | g |
| KH2PO4 | 0.3 | g |
| Casitone (BD-Difco) | 1.0 | g |
| Yeast extract | 0.5 | g |
| A5 solution (see below) | 1.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Autoclave under a N2 gas atmosphere. After cooling, add per liter the following solution (autoclaved and stored under a N2 gas atmosphere):
| 5% Na2S·9H2O solution | 5.0 | ml |
A5 solution:
| Co(NO3)2·6H2O | 4.94 | mg |
| CuSO4·5H2O | 7.9 | mg |
| H3BO3 | 0.286 | g |
| MnCl2·4H2O | 0.181 | g |
| Na2MoO4·2H2O | 39.0 | mg |
| ZnSO4·7H2O | 22.2 | mg |
| Distilled water | 1.0 | L |
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