Medium data

Search for medium no. = [964].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

964   480G MEDIUM

NaCl  0.5     g
NH4Cl  0.33    g
CaCl2·2H2O  0.15    g
MgCl2·6H2O  0.35    g
KCl  0.3     g
KH2PO4  0.3     g
Casitone (BD-Difco)  1.0     g
Yeast extract  0.5     g
A5 solution (see below)  1.0     g
Resazurin  1.0     mg
Distilled water  1.0     L
Autoclave under a N2 gas atmosphere. After cooling, add per liter the following solution (autoclaved and stored under a N2 gas atmosphere):
5% Na2S·9H2O solution  5.0     ml
A5 solution:
Co(NO3)2·6H2O  4.94    mg
CuSO4·5H2O  7.9     mg
H3BO3  0.286   g
MnCl2·4H2O  0.181   g
Na2MoO4·2H2O  39.0     mg
ZnSO4·7H2O  22.2     mg
Distilled water  1.0     L