Medium data
Search for medium no. = [959].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
959 THERMANAEROBACTER THERMOPHILA MEDIUM
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NH4Cl | 0.3 | g |
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.5 | g |
| CaCl2·2H2O | 0.15 | g |
| KCl | 0.1 | g |
| Trace element solution (see Medium No. 294) | 1.0 | ml |
| Yeast extract | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 935.0 | ml |
Mix components thoroughly, adjust pH to 7.2 with KOH, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 935 ml of the medium) from anaerobic stocks (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 25.0 | ml |
| 1 M Glucose solution | 20.0 | ml |
| 1 M Na2S2O3·5H2O* | 20.0 | ml |
| 5% Na2S·9H2O solution | 8.0 | ml |
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved