Medium data

Search for medium no. = [959].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

959   THERMANAEROBACTER THERMOPHILA MEDIUM

KH2PO4  0.3     g
K2HPO4  0.3     g
NH4Cl  0.3     g
NaCl  1.0     g
MgCl2·6H2O  0.5     g
CaCl2·2H2O  0.15    g
KCl  0.1     g
Trace element solution (see Medium No. 294)  1.0     ml
Yeast extract  0.5     g
Resazurin  1.0     mg
Distilled water  935.0     ml
Mix components thoroughly, adjust pH to 7.2 with KOH, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 935 ml of the medium) from anaerobic stocks (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
1 M Glucose solution  20.0     ml
1 M Na2S2O3·5H2O*  20.0     ml
5% Na2S·9H2O solution  8.0     ml