Medium data

Search for medium no. = [954].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

954   NEUTRAL OLIGOTROPHIC HALOARCHAEAL MEDIUM NO.3

MDS salt water (see Medium No. 574)  767.0     ml
Yeast extract  1.0     g
Fish peptone (Fluka)  0.25    g
Sodium formate  0.25    g
Sodium acetate  0.25    g
Sodium lactate  0.25    g
Sodium pyruvate  0.25    g
Distilled water  233.0     ml
Mix components thoroughly and adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar. (if necessary, use washed agar as described in the recipe of Medium No. 574). Autoclave and, after cooling, add the following solutions (*filter-sterilized or autoclaved) to the medium.
1 M NH4Cl solution*  5.0     ml
Potassium phosphate buffer (see Medium No. 574)  2.0     ml