Medium data

Search for medium no. = [954].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

954 NEUTRAL OLIGOTROPHIC HALOARCHAEAL MEDIUM NO.3

MDS salt water (see Medium No. 574)	`767.0`	ml
Yeast extract	`1.0`	g
Fish peptone (Fluka)	`0.25`	g
Sodium formate	`0.25`	g
Sodium acetate	`0.25`	g
Sodium lactate	`0.25`	g
Sodium pyruvate	`0.25`	g
Distilled water	`233.0`	ml

Mix components thoroughly and adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar. (if necessary, use washed agar as described in the recipe of Medium No. 574). Autoclave and, after cooling, add the following solutions (*filter-sterilized or autoclaved) to the medium.

1 M NH₄Cl solution*	`5.0`	ml
Potassium phosphate buffer (see Medium No. 574)	`2.0`	ml