Medium data
Search for medium no. = [948].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
948 MODIFIED BALCH'S METHANOGEN MEDIUM 1/B
| KH2PO4 | 0.3 | g |
| K2HPO4 | 0.3 | g |
| NH4Cl | 0.3 | g |
| NaCl | 0.6 | g |
| MgCl2·6H2O | 0.13 | g |
| FeSO4·7H2O | 2.0 | mg |
| CaCl2·2H2O | 8.0 | mg |
| Yeast extract (BD-Difco) | 0.2 | g |
| Sodium acetate | 2.5 | g |
| Sodium formate | 2.5 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| NaHCO3 | 5.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients, except NaHCO3, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20, v/v), and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., serum bottles, Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand the autoclaved medium overnight. Prior to inoculation, add per liter the following solutions aseptically and anaerobically (autoclaved and stored under a N2 atmosphere):
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (80:20, v/v).
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