Medium data

Search for medium no. = [946].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

946   KORTHOF'S MEDIUM

10 x Korthof's basal medium (see below)  50.0     ml
Rabbit serum heat-inactivated at 56℃ for 30 min  50.0     ml
Distilled water  400.0     ml
Autoclave distilled water. After autoclaving, allow to cool, aseptically add 10 x Korthof's basal medium and heat-inactivated rabbit serum that has been filter-sterilized ahead of time.
10 x Korthof's basal medium:
NaCl  7.0     g
Na2HPO4  4.4     g
Polypeptone peptone (BD-BBL)  4.0     g
KH2PO4  1.2     g
NaHCO3  0.1     g
KCl  0.2     g
CaCl2·2H2O  0.2     g
Distilled water  450.0     ml
Mix ingredients except CaCl2 and autoclave. Dissolve CaCl2 in 50 ml distilled water and filter-sterilize. After autoclaving, allow to cool, add sterile CaCl2 solution to the medium.