Medium data

Search for medium no. = [920].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

920 HYDROGENISPORA MEDIUM

Yeast extract (BD-Difco)	`0.2`	g
KH₂PO₄	`0.14`	g
MgCl₂·6H₂O	`0.2`	g
CaCl₂·2H₂O	`0.15`	g
NH₄Cl	`0.54`	g
Trace vitamins solution (see Medium No. 284)	`1.0`	ml
Trace element solution (see Medium No. 284)	`1.0`	ml
Se/W solution (see Medium No. 284)	`1.0`	ml
NaHCO₃	`2.5`	g
Resazurin	`1.0`	mg
Distilled water	`1.0`	L

Mix components, except NaHCO₃, and adjust pH to 6.5. Bring to a boil for 5-10 sec, cool down under a stream of a N₂-CO₂ (80:20 v/v) gas mixture and add NaHCO₃ to the solution. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottle) under the same gas mixture and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Then, add per liter the following solutions:

1.0 M Glucose solution	`10.0`	ml
5% L-Cysteine·HCl·H₂O solution	`6.0`	ml
5% Na₂S·9H₂O solution	`6.0`	ml