Medium data

Search for medium no. = [918].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

918 DESULFAMPLUS MEDIUM

NaCl	`20.0`	g
MgCl₂·6H₂O	`6.0`	g
Na₂SO₄	`3.24`	g
CaCl₂·2H₂O	`1.0`	g
KCl	`0.55`	g
NH₄Cl	`0.3`	g
Modified Wolfe's mineral solution (see Medium No. 915)	`5.0`	ml
Sodium fumarate	`1.0`	g
HEPES	`2.4`	g
Yeast extract	`0.2`	g
Resazurin	`0.4`	mg
Distilled water	`1.0`	L

Mix components thoroughly and adjust pH to 7.0 with NaOH. Distribute the medium into culture vessels under a stream of a N₂ gas, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions from anaerobic stock solutions (autoclaved or *filter-sterilized):

0.5 M Potassium phosphate buffer (pH 7.0)	`1.8`	ml
Vitamin solution* (see Medium No. 915)	`0.5`	ml
8% NaHCO₃ solution*	`2.35`	ml
5% L-Cysteine·HCl·H₂O solution (pH 7.0)	`8.0`	ml
0.01 M FeSO₄·5H₂Osolution (in 0.02 N HCl) (optional)	`10.0`	ml

Readjust pH to 7.2 - 7.5, if necessary, with sterile HCl or NaOH solution. Inoculate when the medium turns colorless.
Comment: Optionally add the FeSO₄·5H₂O solution as described, for the formation of magnetosome.