Medium data

Search for medium no. = [896].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

896 MODIFIED BSK MEDIUM

HEPES	`3.0`	g
Trisodium citrate·2H₂O	`0.35`	g
Glucose	`2.5`	g
Sodium pyruvate	`0.4`	g
N-Acetyl-D-glucosamine	`0.2`	g
NaHCO₃	`1.1`	g
MEM alpha Modification (BioWest)	`5.2`	g
TC Yeastolate (BD-Difco)	`1.0`	g
Probumin Universal Grade (Millipore)	`25.0`	g
Neopeptone (BD-Difco)	`2.5`	g
Rabbit serum sterile non-hemolyzed young (Pel-Freez code No. 31125)	`40.0`	ml

Adjust pH to 7.2 with NaOH, fill up to 584 ml with distilled water and filter-sterilize with 0.22 µm PES filter.