Medium data
Search for medium no. = [893].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
893 MBBS MEDIUM FOR HYDROGENOBACTER
| Modified Brock's salt base solution (see below) | 1.0 | L |
| 10% Yeast extract solution | 5.0 | ml |
| 8% NaHCO3 solution (filter-sterilized) | 10.0 | ml |
| Sulfur (powder) | 1.0 | g |
Autoclave the base solution and the yeast extract solution separately. Filter-sterilize the NaHCO3 solution and steam sulfur for 3 hr on each of 3 successive 3 days. Aseptically mix the three solutions and distribute into culture vessels containing sulfur under a H2-CO2-O2 (80:20:2, v/v) gas stream and seal with butyl rubber stoppers. Adjust pH to 6.0 with sterile 1 N H2SO4, if necessary. Pressurize inoculated bottles to 100 kPa with the same gas mixture.
Modified Brock's salt base solution:
| (NH4)2SO4 | 1.3 | g |
| KH2PO4 | 0.28 | g |
| MgSO4·7H2O | 0.25 | g |
| CaCl2·2H2O | 0.07 | g |
| FeCl3·6H2O | 2.0 | mg |
| MnCl2·4H2O | 1.8 | mg |
| Na2B4O7·10H2O | 4.5 | mg |
| ZnSO4·7H2O | 0.22 | mg |
| CuCl2·2H2O | 0.05 | mg |
| Na2MoO4·2H2O | 0.03 | mg |
| VOSO4·xH2O | 0.03 | mg |
| CoSO4·7H2O | 0.01 | mg |
| Distilled water | 1.0 | L |
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