Medium data

Search for medium no. = [891].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

891   RAE MEDIUM

Glucose  40.0     g
Peptone  10.0     g
Yeast extract  10.0     g
Citric acid  1.5     g
Na2HPO4·2H2O  3.38    g
Glacial acetic acid  10.0     ml
Ethanol  10.0     ml
Agar  20.0     g
Distilled water  980.0     ml
Separately filter-sterilize glacial acetic acid and ethanol (use Teflon filters), and aseptically add to the sterile medium. Final pH should be about 3.8.