Medium data

Search for medium no. = [89].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

89   SM MEDIUM

KH2PO4  1.5     g
Na2HPO4  4.5     g
NH4Cl  0.3     g
MgSO4·7H2O  0.1     g
10% Na2S2O3 solution  100.0     ml
Trace metal solution (see below)  5.0     ml
Distilled water  900.0     ml
Adjust pH to 7.5.
Separately sterilize Na2S2O3 solution by filtration. Aseptically add sterile Na2S2O3 solution to the medium.
Trace metal solution:
EDTA  50.0     g
ZnSO4·7H2O  22.0     g
CaCl2  5.54    g
MnCl2·4H2O  5.06    g
FeSO4·7H2O  4.99    g
(NH4)6Mo7O24·4H2O  1.10    g
CuSO4·5H2O  1.57    g
CoCl2·6H2O  1.61    g
Distilled water  1.0     L
Adjust pH to 6.0 with KOH.