Medium data
Search for medium no. = [89].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
89 SM MEDIUM
| KH2PO4 | 1.5 | g |
| Na2HPO4 | 4.5 | g |
| NH4Cl | 0.3 | g |
| MgSO4·7H2O | 0.1 | g |
| 10% Na2S2O3 solution | 100.0 | ml |
| Trace metal solution (see below) | 5.0 | ml |
| Distilled water | 900.0 | ml |
Adjust pH to 7.5.
Separately sterilize Na2S2O3 solution by filtration. Aseptically add sterile Na2S2O3 solution to the medium.
Trace metal solution:
| EDTA | 50.0 | g |
| ZnSO4·7H2O | 22.0 | g |
| CaCl2 | 5.54 | g |
| MnCl2·4H2O | 5.06 | g |
| FeSO4·7H2O | 4.99 | g |
| (NH4)6Mo7O24·4H2O | 1.10 | g |
| CuSO4·5H2O | 1.57 | g |
| CoCl2·6H2O | 1.61 | g |
| Distilled water | 1.0 | L |
Adjust pH to 6.0 with KOH.
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