Medium data
Search for medium no. = [886].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
886 MMA MEDIUM
| (NH4)2SO4 | 1.0 | g |
| K2HPO4 | 0.5 | g |
| NaHCO3 | 0.5 | g |
| Trace element solution see below) | 10.0 | ml |
| HEPES | 11.9 | g |
Add components to distilled water and bring volume to 1.0 L.
Adjust pH to 7.8.
Trace element solution:
| MgSO4·7H2O | 5.0 | g |
| CaCl2·2H2O | 0.5 | g |
| CuSO4·5H2O | 0.01 | g |
| MnSO4·xH2O | 0.2 | g |
| EDTA·Fe(III) | 0.5 | g |
| Na2MoO4·2H2O | 5.0 | mg |
| CoCl2·6H2O | 0.1 | mg |
| ZnSO4·7H2O | 0.01 | g |
| Distilled water | 1.0 | L |
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