Medium data

Search for medium no. = [88].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Solution I:
Sodium glutamate  10.0     g
KH2PO4  10.0     g
Distilled water  1.0     L
Solution II:
Glycerol  6.0     ml
2% Malachite green solution  6.0     ml
Whole egg  200.0     ml
Homogenize Solution II by stirring and filter through gauze. Mix solutions I and II, and sterilize at 90C for 60 min.
Comment: Commercial 1% Ogawa medium (Eiken or Nissui) is also available.

Copyright © 2023 Microbe Division (JCM) - All Rights Reserved