Medium data

Search for medium no. = [88].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

88   1% OGAWA MEDIUM

Solution I:
Sodium glutamate  10.0     g
KH2PO4  10.0     g
Distilled water  1.0     L
Solution II:
Glycerol  6.0     ml
2% Malachite green solution  6.0     ml
Whole egg  200.0     ml
Homogenize Solution II by stirring and filter through gauze. Mix solutions I and II, and sterilize at 90C for 60 min.
Comment: Commercial 1% Ogawa medium (Eiken or Nissui) is also available.



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