Medium data
Search for medium no. = [88].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
88 1% OGAWA MEDIUM
Solution I:
| Sodium glutamate | 10.0 | g |
| KH2PO4 | 10.0 | g |
| Distilled water | 1.0 | L |
Solution II:
| Glycerol | 6.0 | ml |
| 2% Malachite green solution | 6.0 | ml |
| Whole egg | 200.0 | ml |
Homogenize Solution II by stirring and filter through gauze. Mix solutions I and II, and sterilize at 90C for 60 min.
Comment: Commercial 1% Ogawa medium (Eiken or Nissui) is also available.
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