Medium data

Search for medium no. = [868].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

868   GEOMONAS SACCHAROVORANS MEDIUM

KH2PO4  0.3     g
K2HPO4  0.3     g
NH4Cl  1.0     g
NaCl  1.0     g
KCl  0.1     g
CaCl2·2H2O  0.1     g
Na2SO4  3.0     g
Sodium acetate  1.0     g
Trace element solution (see Medium No. 439)  1.0     ml
Yeast extract  1.0     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  0.5     mg
Distilled water  950.0     ml
Mix components thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 950 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
1 M Fructose solution  20.0     ml
Vitamin solution (see Medium No. 403)*  1.0     ml
Thiamine solution (see Medium No. 403)*  1.0     ml
Vitamin B12 solution (see Medium No. 403)*  1.0     ml
5% Na2S·9H2O solution  8.0     ml