Medium data
Search for medium no. = [848].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
848 MODIFIED 1/10 PYGV MEDIUM
| Solution I (see below) | 75.0 | ml |
| Solution II (see below) | 75.0 | ml |
| Trypticase peptone | 1.0 | g |
| Yeast extract | 0.5 | g |
| Glucose | 10.0 | g |
| 100 x Vitamin mixture (see below) | 1.0 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 7.0. Cultivate under a N2 (100%) atmosphere.
Solution I:
| K2HPO4 | 6.0 | g |
| Distilled water | 1.0 | L |
Solution II:
| KH2PO4 | 6.0 | g |
| NH4Cl | 4.85 | g |
| NaCl | 12.0 | g |
| MgCl2·6H2O | 1.0 | g |
| CaCl2·2H2O | 1.2 | g |
| Distilled water | 1.0 | L |
100 x Vitamin mixture:
| Biotin | 0.1 | g |
| VitaminB12 | 0.1 | g |
| p-Aminobenzoic acid | 0.3 | g |
| Folic acid | 0.5 | g |
| Thiamine·HCl | 0.5 | g |
| Riboflavin | 0.5 | g |
| Pyridoxine·HCl | 1.5 | g |
| Distilled water | 1.0 | L |
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