Medium data

Search for medium no. = [848].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

848   MODIFIED 1/10 PYGV MEDIUM

Solution I (see below)  75.0     ml
Solution II (see below)  75.0     ml
Trypticase peptone  1.0     g
Yeast extract  0.5     g
Glucose  10.0     g
100 x Vitamin mixture (see below)  1.0     ml
Distilled water  1.0     L
Adjust pH to 7.0. Cultivate under a N2 (100%) atmosphere.
Solution I:
K2HPO4  6.0     g
Distilled water  1.0     L
Solution II:
KH2PO4  6.0     g
NH4Cl  4.85    g
NaCl  12.0     g
MgCl2·6H2O  1.0     g
CaCl2·2H2O  1.2     g
Distilled water  1.0     L
100 x Vitamin mixture:
Biotin  0.1     g
VitaminB12  0.1     g
p-Aminobenzoic acid  0.3     g
Folic acid  0.5     g
Thiamine·HCl  0.5     g
Riboflavin  0.5     g
Pyridoxine·HCl  1.5     g
Distilled water  1.0     L