Medium data

Search for medium no. = [846].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

846 MO-CFXI MEDIUM

Solution A:

NaCl	`25.0`	g
KH₂PO₄	`0.1`	g
NH₄Cl	`0.535`	g
NaHCO₃	`2.0`	g
Yeast extract	`1.0`	g
Tryptone (BD-Difco)	`1.0`	g
Glucose	`1.8`	g
Sodium pyruvate	`0.55`	g
Trace vitamins solution (see Medium No. 284)	`2.0`	ml
Trace element solution (see Medium No. 284)	`1.0`	ml
Se/W solution (see Medium No. 284)	`1.0`	ml
Resazurin	`1.0`	mg
Distilled water	`900.0`	ml

Mix components and adjust pH to 7.0. Autoclave under a N₂-CO₂ (80:20, v/v) gas mixture and stand for overnight.
Solution B:

CaCl₂·2H₂O	`1.0`	g
MgCl₂·6H₂O	`4.0`	g
Distilled water	`100.0`	ml

Mix components and autoclave under a N₂ atmosphere.
To complete the medium, mix Solutions A and B aseptically and anaerobically under a N₂-CO₂ (80:20, v/v) gas mixture. Distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml medium in 50 ml serum bottle) and seal with butyl rubber stoppers. Finally add per liter the following solutions (autoclaved and stored under a N₂ atmosphere):

3% Na₂S·9H₂O solution	`10.0`	ml
3% L-Cysteine·HCl·H₂O solution	`10.0`	ml