Medium data
Search for medium no. = [820].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
820 METHANOTROPH 1A MEDIUM
| NaNO3 | 1.0 | g |
| MgSO4·7H2O | 0.1 | g |
| NaHPO4·12H2O | 0.4 | g |
| KH2PO4 | 0.22 | g |
| CaCl2·2H2O | 0.03 | g |
| FeSO4·7H2O | 2.0 | mg |
| Trace metal solution (see below) | 1.0 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 6.5.
Distribute the medium into culture vessels (leave >80% volume gas phase), seal with butyl rubber stoppers and autoclave. After cooling, add filter-sterilized methane gas to make a final concentration about 20% (v/v) methane in the gas phase.
Trace mineral solution:
| ZnSO4·7H2O | 0.44 | g |
| CuSO4·5H2O | 0.20 | g |
| MnSO4·xH2O | 0.17 | g |
| Na2MoO4·2H2O | 0.06 | g |
| H3BO3 | 0.10 | g |
| CoCl2·6H2O | 0.08 | g |
| Distilled water | 1.0 | L |
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