Medium data

Search for medium no. = [820].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

820   METHANOTROPH 1A MEDIUM

NaNO3  1.0     g
MgSO4·7H2O  0.1     g
NaHPO4·12H2O  0.4     g
KH2PO4  0.22    g
CaCl2·2H2O  0.03    g
FeSO4·7H2O  2.0     mg
Trace metal solution (see below)  1.0     ml
Distilled water  1.0     L
Adjust pH to 6.5.
Distribute the medium into culture vessels (leave >80% volume gas phase), seal with butyl rubber stoppers and autoclave. After cooling, add filter-sterilized methane gas to make a final concentration about 20% (v/v) methane in the gas phase.
Trace mineral solution:
ZnSO4·7H2O  0.44    g
CuSO4·5H2O  0.20    g
MnSO4·xH2O  0.17    g
Na2MoO4·2H2O  0.06    g
H3BO3  0.10    g
CoCl2·6H2O  0.08    g
Distilled water  1.0     L