Medium data

Search for medium no. = [79].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

79   METHANOL-UTILIZING BACTERIA MEDIUM B

Methanol  10.0     ml
(NH4)2SO4  3.0     g
KH2PO4  1.4     g
Na2HPO4  3.0     g
MgSO4·7H2O  0.2     g
Ferric citrate  30.0     mg
CaCl2·2H2O  30.0     mg
MnCl2·4H2O  5.0     mg
ZnSO4·7H2O  5.0     mg
CuSO4·5H2O  0.5     mg
Thiamine·HCl (see below)  0.4     mg
Distilled water  1.0     L
Thiamine·HCl can be replaced by 0.2 g of yeast extract.
Adjust pH to 7.1.