Medium data

Search for medium no. = [781].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

781   CLOSTRIDIUM ACETATE 1 MEDIUM

KH2PO4  0.3     g
K2HPO4  0.3     g
NH4Cl  1.0     g
NaCl  1.0     g
KCl  0.1     g
CaCl2·2H2O  0.1     g
MgCl2·6H2O  0.5     g
Trace element solution (see Medium No. 439)  10.0     ml
Yeast extract  1.0     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
1 M Fructose solution  20.0     ml
5% Na2S·9H2O solution  8.0     ml