Medium data

Search for medium no. = [776].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

776   MH1 MEDIUM FOR SALIARCHAEUM RUBRODURUM

L-Glutamic acid  2.0     g
Trisodium citrate·2H2O  2.0     g
KH2PO4  1.0     g
NH4Cl  1.0     g
K2SO4  5.0     g
MgCl2·6H2O  20.0     g
NaCl  200.0     g
Casamino acids (BD-Difco)  4.0     g
Yeast extract (BD-Difco)  2.0     g
0.2% FeSO4·6H2O solution  2.0     ml
Trace metal solution  2.0     ml
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 5.0 with KOH.
For preparation of solid medium, add the components listed above (reduce the amount of NaCl to 150.0 g) to distilled water and bring volume to 800.0 ml. Separately prepare the following solution:
NaCl  50.0     g
Agar  20.0     g
Distilled water  200.0     ml
Combine both solutions after autoclaving.
Trace metal solution:
CoCl2·6H2O  0.3     g
MnCl2·4H2O  0.1     g
ZnCl2  0.1     g
H3BO4  0.02    g
Na2MoO4·2H2O  0.10    g
NiCl2·6H2O  0.10    g
CuCl2·2H2O  0.01    g
AlCl3  0.04    g
Na2WO4·2H2O  0.02    g
CaCl2·2H2O  1.0     g
BaCl2·2H2O  0.1     g
Na2S2O3·5H2O  2.0     g
Distilled water  1.0     L
Adjust pH to 4.0 with HCl.