Medium data
Search for medium no. = [776].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
776 MH1 MEDIUM FOR SALIARCHAEUM RUBRODURUM
| L-Glutamic acid | 2.0 | g |
| Trisodium citrate·2H2O | 2.0 | g |
| KH2PO4 | 1.0 | g |
| NH4Cl | 1.0 | g |
| K2SO4 | 5.0 | g |
| MgCl2·6H2O | 20.0 | g |
| NaCl | 200.0 | g |
| Casamino acids (BD-Difco) | 4.0 | g |
| Yeast extract (BD-Difco) | 2.0 | g |
| 0.2% FeSO4·6H2O solution | 2.0 | ml |
| Trace metal solution | 2.0 | ml |
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 5.0 with KOH.
For preparation of solid medium, add the components listed above (reduce the amount of NaCl to 150.0 g) to distilled water and bring volume to 800.0 ml. Separately prepare the following solution:
| NaCl | 50.0 | g |
| Agar | 20.0 | g |
| Distilled water | 200.0 | ml |
Combine both solutions after autoclaving.
Trace metal solution:
| CoCl2·6H2O | 0.3 | g |
| MnCl2·4H2O | 0.1 | g |
| ZnCl2 | 0.1 | g |
| H3BO4 | 0.02 | g |
| Na2MoO4·2H2O | 0.10 | g |
| NiCl2·6H2O | 0.10 | g |
| CuCl2·2H2O | 0.01 | g |
| AlCl3 | 0.04 | g |
| Na2WO4·2H2O | 0.02 | g |
| CaCl2·2H2O | 1.0 | g |
| BaCl2·2H2O | 0.1 | g |
| Na2S2O3·5H2O | 2.0 | g |
| Distilled water | 1.0 | L |
Adjust pH to 4.0 with HCl.
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