Medium data

Search for medium no. = [770].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

770   BETAINE+BICARBONATE BUFFERED MEDIUM

Solution A:
Phosphate solution (see below)  15.0     ml
Na2SeO3·5H2O  0.3     mg
Na2WO4·2H2O  0.3     mg
Yeast extract  0.2     g
Resazurin  0.5     mg
Distilled water  900.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture.
Solution B:
Trace metal solution (see below)  1.0     ml
Vitamin solution (see below)  1.0     ml
Mineral salt solution (see below)  12.5     ml
1.0 M Betaine solution  10.0     ml
Distilled water  25.5     ml
Mix components thoroughly and filter-sterilize. Stock the solution under a N2-CO2 (80:20, v/v) gas mixture.
Solution C:
NaHCO3  4.0     g
Na2S·9H2O  0.25    g
L-Cysteine·HCl·H2O  0.5     g
Distilled water  50.0     ml
Mix components thoroughly and filter-sterilize. Stock the solution under a N2-CO2 (80:20, v/v) gas mixture.
To complete the medium, combine Solutions A, B and C aseptically and anaerobically under a N2-CO2 (80:20, v/v) gas mixture. Check the final pH to be 6.9 - 7.2.
Phosphate solution:
KH2PO4  0.41    g
Na2HPO4  0.43    g
Distilled water  100.0     ml
Trace metal solution:
FeCl2·4H2O  2.0     g
H3BO3  0.05    g
ZnCl2  0.05    g
CuCl2·2H2O  0.03    g
MnCl2·4H2O  0.5     g
Na2MoO4·2H2O  0.05    g
AlCl3  0.05    g
CoCl2·6H2O  0.05    g
NiCl2·H2O  0.05    g
EDTA  0.5     g
32% HCl  1.0     ml
Distilled water  1.0     L
Mineral salt solution:
NH4Cl  24.0     g
NaCl  24.0     g
CaCl2·2H2O  8.8     g
MgCl2·6H2O  8.0     g
Distilled water  1.0     L
Vitamin solution:
Biotin  0.02    g
Folic acid  0.02    g
Pyridoxamine  0.25    g
Pyridoxine·HCl  0.1     g
Thiamine·HCl  0.1     g
Riboflavin  0.05    g
Nicotinic acid  0.1     g
Nicotinamide  0.1     g
DL-Calcium pantothenate  0.05    g
Vitamin B12  0.05    g
p-Aminobenzoic acid  0.05    g
Lipoic acid  0.05    g
Distilled water  1.0     L