Medium data
Search for medium no. = [766].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
766 SYNBIOBACTERIUM KY46 MEDIUM
| Tryptone (BD-Difco) | 10.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| KH2PO4 | 1.6 | g |
| NaCl | 5.0 | g |
| NaNO3 | 1.7 | g |
| Distilled water | 1.0 | L |
Mix components and adjust pH to 8.0 with NaOH. Distribute the medium into culture vessels, replace the gas phase with a N2 gas and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (filter-sterilized):
| 2.0 M NH4HCO3 solution | 20.0 | ml |
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved