Medium data

Search for medium no. = [766].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Tryptone (BD-Difco)  10.0     g
Yeast extract (BD-Difco)  5.0     g
KH2PO4  1.6     g
NaCl  5.0     g
NaNO3  1.7     g
Distilled water  1.0     L
Mix components and adjust pH to 8.0 with NaOH. Distribute the medium into culture vessels, replace the gas phase with a N2 gas and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (filter-sterilized):
2.0 M NH4HCO3 solution  20.0     ml

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