Medium data
Search for medium no. = [745].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
745 MJYP2 MEDIUM
| MJ(-N) synthetic seawater (see Medium No. 268) | 1.0 | L |
| NaNO3 | 0.5 | g |
| NH4Cl | 0.25 | g |
| Yeast extract (BD-Difco) | 1.0 | g |
| Tryptone (BD-Difco) | 1.0 | g |
| Trace vitamin (see Medium No. 197) | 10.0 | ml |
Mix components, adjust pH to 7.0 and autoclave. Replace the gas phase with N2-O2 (99:1, v/v) gas mixture, and pressurize to 200 kPa with the same gas mixture.
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